Fig. 8
From: Cullin-7 (CUL7) is overexpressed in glioma cells and promotes tumorigenesis via NF-κB activation
MiR-3940-5p inhibits cell proliferation, migration and invasion of glioma. U87MG and U251 cells transfected with miR-3940-5p mimics or controls and characterized in the following assays: (a, b) EdU performed 48 h after transfection (scale bar = 100 μm); (c) growth curve based on OD450 using the CCK-8 assay; (d, f) cell cycle profiles determined from PI staining in flow cytometry (e, g) % apoptosis as determined with Annexin V-FITC antibody and PI staining in flow cytometry. (h) Representative images of transwell migration and invasion assays performed in U87MG and U251 transfected NC and miR-3940-5p cells. Graphic representation of migrated and invaded cells counts from transwell assay (Scale bar = 200 μm). Data are represented as the mean ± SEM from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001, relative to control. NC: negative control RNA; miR-3940-5p: miR-3940-5p mimics