Fig. 3

Silencing circFOXM1 influences expression of cell cycle-related genes. a A volcano plot illustrated 685 differentially expressed genes in H1299 cells after silencing circFOXM1 by RNA sequencing analysis (|Fold change| > 2, FDR < 0.1). b A cluster heat map showed all 685 differentially expressed genes in H1299 cells after silencing circFOXM1. c Gene Set Enrichment Analysis (GSEA) indicated that the signatures of cell_cycle, cell_cycle_mitotic, mitotic G2_G2_M phase, and G1 phase were significantly enriched in circFOXM1 silencing group compared to control. NES: Normalized Enrichment Scores. d Validation of 6 cell cycle-related genes by qRT-PCR in H1299 and H2170 cells with circFOXM1 silencing. *P < 0.05