Fig. 4

circFOXM1 facilitates cell proliferation by sponging miR-614. a RNA fluorescence in situ hybridization (FISH) showed that circFOXM1 was predominantly localized in cytoplasm in H1299 cells. U6 was mainly localized in nucleus. 18S was mainly localized in cytoplasm. b Cytoplasmic and nuclear RNA fractionation experiments showed that circFOXM1 was mainly located in the cytoplasm in H1299 cells. c Ago2 RIP assay was used for the detection of circFOXM1 in H1299 and H2170 cells expressing Flag-Ago2 or Flag-tag. d The putative binding sites of 17 predicted miRNAs on circFOXM1. e RNA pull-down assay was used for the detection of circFOXM1 by circFOXM1 specific probe. f-g Relative expression of circFOXM1 putative binding miRNAs was examined by qRT-PCR analysis by circFOXM1 specific probe. h Luciferase activity of luc-circFOXM1 wt or Luc-circFOXM1 mut in H1299 and H2170 cells co-transfected with miR-614 mimics. i-j Cell proliferation analysis of H1299 and H2170 cells transfected with control, circFOXM1, circFOXM1 + miR-NC (miRNA control), or circFOXM1 + miR-614. *P < 0.05; **P < 0.01