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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Circular RNA hsa_circ_0000326 acts as a miR-338-3p sponge to facilitate lung adenocarcinoma progression

Fig. 3

Hsa_circ_0000326 acted as a sponge of miR-338-3p. (a) Putative targets of hsa_circ_0000326 were predicted using custom Arraystar software. (b) Effects of five predicted miRNAs and a negative control on the luciferase activity of hsa_circ_0000326, as determined by luciferase reporter assay. (c) The results of the luciferase reporter assay validated the interaction between hsa_circ_0000326 and miR-338-3p. (d) Colocalization between hsa_circ_0000326 and miR-338-3p was observed (arrowheads) using RNA-FISH in A549 cells. The nuclei were stained with DAPI. Scale bar = 40 μm. (e) RNA pulldown assay in A549 cells showed hsa_circ_0000326 could interact with miR-338-3p. The bars with error bars represent the mean ± SD from three independent experiments. *P < 0.05

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