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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Circular RNA hsa_circ_0000326 acts as a miR-338-3p sponge to facilitate lung adenocarcinoma progression

Fig. 5

Hsa_circ_0000326 inhibited the posttranscriptional activity of miR-338-3p and upregulated the miR-338-3p target RAB14. (a) Expression of miR-338-3p in cells transfected with hsa_circ_0000326 overexpression plasmids or siRNAs. (b) Expression of SOX4, ZEB2 and RAB14 in miR-338-3p-expressing or miR-338-3p-depleted cells, as determined by western blot analysis. The expression levels were normalized to GAPDH expression levels. (c) Expression of SOX4, ZEB2 and RAB14 in cells transfected with the hsa_circ_0000326 overexpression plasmid (pAV-circ0000326) and miR-338-3p mimics. The bars with error bars represent the mean ± SD from three independent experiments. (d) Images of the xenograft tumors in each group 3 weeks after subcutaneous implantation of H1299 cells stably infected with hsa_circ_0000326-knockdown lentivirus (LV-si-circ0000326) or control lentivirus (LV-NC) (n = 7). (e) Effect of hsa_circ_0000326 knockdown on tumor weight 3 weeks after subcutaneous injection of H1299 cells stably infected with LV-si-circ0000326 or LV-NC. (f) Effect of intratumoral hsa_circ_0000326 knockdown on tumor growth in BALB/c nude mice. *P < 0.05

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