Fig. 3

Oncogenic potential of RET mutants in vitro and in vivo. a Lysates from epithelial ovarian cancer cell (EOC) A2780 transduced with EV or WT or mutants were analyzed by immunoblot with anti-phospho RET (Y905), anti-RET antibody, anti-phospho ERK (T202/204), anti-ERK antibody, anti-phospho AKT (S473), and anti-AKT antibody (b-d) Bar graphs showing quantification of western blot bands in (a), normalized to WT control. e R693H and A750T mutants increase the cell viability of A2780 cells. A2780 cells stably expressing EV, RET WT or RET mutants grew on 96-well plates in triplicate for 72 h, and the viability was measured by CTG assay. f and g A2780 cells transduced with EV, RET WT or mutants were seeded in 6-well plates (500 cells per well) in triplicate for 2 weeks and stained with MTT. Representative plates (f) and number of colonies are presented (g). h–j RET mutants promote the growth of ovarian cancer xenografts in nude mice. Representative pictures (h), tumor volumes (i) and tumor weights (j) are shown. *, P < 0.05 compared to WT. **, P < 0.01 compared to WT