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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Runt-related transcription factor 1 promotes apoptosis and inhibits neuroblastoma progression in vitro and in vivo

Fig. 1

Identification of RUNX1 as an apoptosis transcription factor and an independent prognostic factor in NB tissue and cell lines. a Cluster analysis of apoptosis genes in the KEGG and ChIP-X databases. We selected the top ten transcription factors (TFs), as shown. b Kaplan-Meier curves indicating the survival of 498 (GES49710, cutoff value = 292.04) NB patients with high or low HNF4A and RUNX1 expression, respectively (Log-rank test, p = 0.053, p = 0019, respectively). c Mining of public microarray datasets (GSE49710) revealing the RUNX1 expression levels in tumors with various MYCN amplification levels, progression and INSS stages. (unpaired t-test, p < 0.0001, p < 0.0001, P = 0.0014.) d and e Western blot and immunohistochemical staining images indicated protein levels of RUNX1 in g ganglioneuroma (GN, control), well differentiated (WD) NB, poor differentiated (PD) NB, and undifferentiated NB tissues (n = 5 per group). Scale bar: 100 μm. ***P < 0.001 versus GN. f Western blot assay indicating the levels of RUNX1 in NB cell lines (SH-SY5Y, SK-N-SH, SK-N-AS, IMR-32, TN-2). Data are shown as mean ± SEM and representative of three independent experiments in panels (d)–(f). Exact P values are specified in Additional file 2: Table S3

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