Fig. 4

RUNX1 promoted apoptosis in NB cells and altered the transcription and protein levels of BIRC5, CSF2RB, and NFKBIA. a Venn diagram heatmap showing the genes correlated with RUNX1 in NB cases public datasets (GSE45547 and GSE49710, p < 0.05) and RUNX1 target genes of apoptosis in KEGG datasets. b Heatmap derived from public datasets (GSE49710) revealing the expression of the RUNX1 target gene (BIRC5, TUBA1A, FOS, NFKBIA, TNFSF10, CSF2RB, and PARP4). c and d Real-time quantitative RT-PCR and western blot indicating the mRNA and protein level of the RUNX1 target gene in NB cells stably transfected with mock, RUNX1, sh-Scb, sh-RUNX1#1 and sh-RUNX1#2. *P < 0.05 vs. mock or sh-Scb, **P < 0.01 vs. mock or sh-Scb, ***P < 0.001 vs. mock or sh-Scb. Data are shown as mean ± SEM and representative of three independent experiments in panels (c)–(d). Exact P values are specified in Additional file 2: Table S3