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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Runt-related transcription factor 1 promotes apoptosis and inhibits neuroblastoma progression in vitro and in vivo

Fig. 5

RUNX1 altered the transcription and protein levels of BIRC5, CSF2RB, and NFKBIA through direct binding of its promoter. a Overview of the RUNX1 motifs found at RUNX1 binding sites. b ChIP and qPCR assays indicating enrichment of RUNX1 on target gene promoters in SH-SY5Y and SK-N-SH cells transfected with mock, RUNX1, sh-Scb, sh-RUNX1#1, and sh-RUNX1#2. c Dual-luciferase assay showing the relative activity (36 h post transfection) of BIRC5, CSF2RB, and NFKBIA promoters in cells stably transfected as indicated. d Kaplan-Meier curves showing the survival of 498 patients (GSE49710) with high or low BIRC5, CSF2RB, and NFKBIA expression (cutoff values = 13,969.57, 151.17 and 19,483.97, respectively). *P < 0.05 vs. mock or sh-Scb, **P < 0.01 vs. mock or sh-Scb, ***P < 0.001 vs. mock or sh-Scb. Data are shown as mean ± SEM and representative of three independent experiments in panels (b)–(c). Exact P values are specified in Additional file 2: Table S3

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