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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Runt-related transcription factor 1 promotes apoptosis and inhibits neuroblastoma progression in vitro and in vivo

Fig. 6

Ectopic expression of RUNX1 suppresses NB cell growth, metastasis, and angiogenesis and promotes apoptosis in vivo. a Timeline (left panel), after tumor cells injection 30 days, athymic nude mice (n = 5) from each group were sacrificed. In vivo growth curves (middle and left panel) of xenografts in athymic nude mice formed by subcutaneous injection of SH-SY5Y cells stably transfected with mock, RUNX1, sh-Scb, or sh-RUNX1#1 into the dorsal flanks (n = 5 per group). b Representative in vivo fluorescence images (left panel) of athymic nude mice bearing xenografts; the weight of xenografts at the end points (right panel) formed by subcutaneous injection of SH-SY5Y cells stably transfected as indicated in nude mice (n = 5 per group). c Representative images (left panel) and quantification (right panel) of immunohistochemical staining showing the expression of Ki-67 and CD31 within xenograft tumors formed by hypodermic injection of SH-SY5Y cells stably transfected as indicated (n = 5 per group). Scale bars: 100 μm. d Western blot assays (normalized to β-actin) showing the expression of RUNX1 and its target genes and apoptosis genes in xenograft tumors formed by subcutaneous injection of SH-SY5Y cells stably transfected as indicated in nude mice into dorsal flanks of athymic nude mice (n = 5 per group). e Timeline (left panel), after tumor cells injection 45 days, athymic nude mice (n = 5) from each group were sacrificed. in vivo imaging of lungs (middle upper panel), metastatic counts of lungs (middle lower panel) and H&E staining images (right panel) of nude mice (n = 5 per group) treated with tail vein injection of SH-SY5Y cells stably transfected as indicated . Scale bar: 100 μm. *P < 0.05 vs. mock or sh-Scb, **P < 0.01 vs. mock or sh-Scb, ***P < 0.001 vs. mock or sh-Scb. Data information: Data are presented as mean ± SEM. Exact P values are specified in Additional file 2: Table S3

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