Fig. 6
CRKL overexpression affects the in vitro migration, invasion and F-actin cytoskeleton expression and arrangement of HCC cells. a CRKL were over-expressed in HepG2, HCCLM3 and HuH7 cells. PCDH-EF1-MCS-T2A-Puro-CRKL or PCDH-EF1-MCS-T2A-Puro were stably transfected into HepG2, HCCLM3 and HuH7 cells, screened against 0.5 μg/ml puromycin. Relative CRKL protein expression levels were determined by WB using GAPDH as internal reference. b and c Transwell assays showed that CRKL overexpression promoted the in vitro migration and invasion abilities of HepG2, HCCLM3 and HuH7 cells. d FITC-phalloidin assays showed that CRKL overexpression increased cytoskeleton F-actin protein expression and filament arrangement of HepG2, HCCLM3 and HuH7 cells