Fig. 8

ETV6 negatively regulates miR-429 by directly binding to its promoter region. a ETV6 downregulation increased miR-429 expression level in CRKL-overexpression HepG2, HCCLM3 and HuH7 cells. b Putative binding site for ETV6 protein at promoter region of miR-429. c ChIP assay of the binding of ETV6 to the promoter region of miR-429. Anti-RNA polymerase II was used as a positive control for binding to the GAPDH promoter region, normal mouse IgG was as a negative control for binding to the GAPDH promoter region, anti-ETV6 was used for experimental group on binding to the miR-429 promoter region, anti-rabbit IgG was used as a control. PCR products were analyzed by 1% agarose gel