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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Long noncoding RNA HOST2, working as a competitive endogenous RNA, promotes STAT3-mediated cell proliferation and migration via decoying of let-7b in triple-negative breast cancer

Fig. 3

HOST2 serves as a let-7b and miR-1266 sponge in TNBC cells. a Seven potential target miRNAs of HOST2 were predicted by miRanda and miRbase. b, c The expression of seven potential target miRNAs in MDA-MB-231 and MDA-MB-468 cells after knockdowns of HOST2. d Schematic model showed the putative binding sites of let-7b and miR-1266 associated with HOST2. e, f Luciferase reporter assay in HEK293 T cells co-transfected with let-7b/miR-1266 mimics, psiCHECK-2-wild type HOST2 (HOST2-wt) or psiCHECk-2-mutant type HOST2 (HOST2-mut) plasmids. g, h RIP binding assay was performed using input from cell lysate, normal mouse IgG or anti-Ago2. Relative expression levels of HOST2 and let-7b/miR-1266 were determined by qRT-PCR. *P < 0.05, **P < 0.01. All experiments were repeated three times

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