Fig. 2

Exosomes increased FUT4 expression and fucosylation of CRC cells not by directly transmitting FUT4 mRNA (a) Glycosyltransferase genes in SW620 and SW480 cells were detected by Microarray analysis. b The expression of glycosyltransferase genes was shown in SW480 cells treated with Exo-SW620. c The expression of glycosyltransferase genes was shown in HCT-8 cells after treatment with Exo-LoVo. d Higher levels of FUT4 were determined in SW480 and HCT-8 cells treated with the exosomes from cultured SW620 or LoVo cells by immunofluorescence staining. e The level of FUT4 was verified by western blot. f, g The altered level of α1, 3-fucosylation was detected by FCM (f) and LTL blotting (g) analysis. h CCK8 assays were conducted to identify the viability of SW480 and HCT-8 cells treated with Exo-siFUT4-SW620 or Exo-siFUT4-LoVo. i Wound healing and transwell assays were used to determine the invasive and migratory ability of treated CRC cells. j FUT4 expression was analyzed in SW480 and HCT-8 cells treated with Exo-siFUT4-SW620 or Exo-siFUT4-LoVo. Data were means ± SD of three independent assays (*P < 0.05, **P < 0.01)