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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Exosomal MALAT1 sponges miR-26a/26b to promote the invasion and metastasis of colorectal cancer via FUT4 enhanced fucosylation and PI3K/Akt pathway

Fig. 5

Exosomal MALAT1 mediated FUT4-associated fucosylation and activated the PI3K/AKT/mTOR pathway in vitro (a) Immunofluorescence staining was used to show the location of fucosylation and FUT4 in SW480 cells treated with Exo-SW620, Exo-siRNA-SW620 or Exo-siMALAT1-SW620. b The extracted proteins of SW480 cells with different exosomes treatment underwent western blot to assess the activity of PI3K/Akt/mTOR pathway. c HCT-8 cells treated with Exo-LoVo, Exo-siRNA-LoVo or Exo-siMALAT1-LoVo exhibited different levels of fucosylation and FUT4. d The activity of PI3K/Akt/mTOR pathway in HCT-8 cells with different exosomes treatment was measured by western blot. e SW480 and HCT-8 cells were planted in plates with or without different amounts of exosomes (2.0 Îĵg, 5.0 Îĵg, 10.0 Îĵg) for 48 h and then the activity of PI3K/Akt/mTOR pathway was measured by western blot. f Western blot was used to measure the activity of PI3K/Akt/mTOR pathway in primary CRC cells treated with 10.0 Îĵg exosomes at 6, 12, 24, and 48 h. g Exosomes treatment promoted the activity of the signaling in SW480 cells, while LY294002 reversed the stimulation

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