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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: PERK/NRF2 and autophagy form a resistance mechanism against G9a inhibition in leukemia stem cells

Fig. 3

Sensitization of AML LSCs, but not HSCs, to BIX-01294-induced apoptosis via PERK inhibition. a, Flow cytometric analysis, based on annexin-V/7-AAD exclusion, of apoptotic fractions after treatment of CD34+-enriched primary AML cells from 16 cases with BIX-01294 (5 μmol/L) in the absence or presence of GSK2606414 (10 μmol/L) for 48 h. Left panel: annexin-V+ 7-AAD+ CD34+ cells; right panel: CD34+CD38 primary LSCs. b, Flow cytometric analysis, based on annexin-V/7-AAD exclusion, of apoptotic fractions after treatment of normal HSCs from five donors with BIX-01294 (5 μmol/L) in the absence or presence of GSK2606414 (10 μmol/L) for 48 h. Left panel: annexin-V+ 7-AAD+ CD34+ cells; right panel: CD34+CD38 primary HSCs. c, Representative scatter plot of CD34+-enriched primary AML cells

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