Fig. 1

Formononetin (Formo) is a candidate natural product that suppresses non-small cell lung cancer (NSCLC) cells. a, The effect of 98 screened compounds on the cell viability of HCC827 cells. HCC827 cells were treated with 3 μM of commercially available natural product library for 24 h. Cell viability was examined by MTS assay. b, The chemical structure of Formo. c, The effect of osimertinib (Osi) on immortalized epithelial cells NL20 and HBE, and a panel of NSCLC cells, cell viability was determined by MTS assay. d, MTS assay analysis of the cell viability of NL20, HBE, and a panel of NSCLC cells with different doses of Formo treatment. e, The effect of Formo on anchorage-independent cell growth of HCC827 cells. HCC827 cells were treated with Formo or Osi, anchorage-independent cell growth was analyzed by soft agar assay. Left, the representative images of colony formation. Right, qualification analysis of the colony number. f, The effect of Formo on anchorage-independent cell growth of NSCLC cells. H3255, H1975, A549, H1299, and HCC827 OR cells were treated with Formo or Osi, anchorage-independent cell growth was analyzed by soft agar assay. ***p < 0.001. ns, not statistically significant. Scale bar, 200 μm