Fig. 5

Suppression of Akt is required for Formo-induced Mcl-1 downregulation. a, HCC827 cells were treated with DMSO control, Formo, PD98059 (MAPK/ERK inhibitor), or LY294002 (PI3K/Akt inhibitor), WCE was subjected to IB analysis. b, HCC827 cells expressing shGFP or shAkt1 were treated with 10 μM Formo for 24 h, WCE was subjected to IB analysis. c, HCC827 cells were transfected with Myr-Akt1 and treated with 10 μM Formo for 24 h, WCE was subjected to IB analysis. d, HCC827 cells were pre-treated with SB216763 (GSK3 inhibitor) or DMSO control for 2 h, followed by Formo treatment for 24 h, WCE was subjected to IB analysis. e, HCC827 cells expressing shGFP or shGSK3β were treated with 10 μM Formo for 24 h, WCE was subjected to IB analysis. f, GSK3β knockout HCC827 cells were transfected with Flag- GSK3β WT or Flag-GSK3β S9A and treated with Formo for 24 h, WCE was subjected to IB analysis