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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Formononetin inhibits tumor growth by suppression of EGFR-Akt-Mcl-1 axis in non-small cell lung cancer

Fig. 6

Formo promotes Mcl-1 ubiquitination in NSCLC cells. a, NSCLC cells were treated with Formo for 24 h, the Mcl-1 RNA level was determined by qRT-PCR. b, NSCLC cells were treated with Formo for 24 h, and incubated with MG132 for another 6 h. WCE was subjected to IB analysis. c, HCC827 cells were treated with/without Formo for 24 h, cycloheximide (CHX) was added into cell culture medium and maintained for various time points, WCE was subjected to IB analysis. d, HCC827 cells were treated with Formo for 24 h, followed by MG132 incubation for another 6 h. WCE was subjected to Mcl-1 ubiquitination analysis. e, Formo promotes Mcl-1 phosphorylation. NSCLC cells were treated with Formo for 24 h, WCE was subjected to IB analysis. f, HCC827 cells were treated with Formo for 24 h, followed by MG132 incubation for another 6 h. WCE was subjected to co-IP assay and IB analysis. G and H, HCC827 cells expressing shGFP or shFBW7 were treated with 10 μM Formo for 24 h, followed by MG132 incubation for another 6 h, WCE was subjected to Mcl-1 ubiquitination analysis (g). The cell viability was examined by MTS assay (h)

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