Fig. 2

Silencing of NCK1-AS1 inhibits the malignant behaviors of U251 cells. a. NCK1-AS1 expression in U251 cells following interventions measured using RT-qPCR; b proliferation of U251 cells detected using EdU assay; c migration of U251 cells detected using the scratch test and observed under the microscope (× 100); d number of invaded cells in the Transwell assay observed under the microscope (× 100); E-F, cell cycle (e) and the apoptosis rate (f) of U251 cells measured using flow cytometry. * compared to the sh-NC group, p < 0.05; ^# compared to the oe-NC group, p < 0.05. Measurement data were measured using mean ± SD; differences among multiple groups were analyzed using one-way ANOVA; Repetition = 3