Fig. 4

NCK1-AS1 acts as a ceRNA in glioma cancer through regulation of miR-138-2-3p. a, sublocalization of NCK1-AS1 in U251 cells detected using FISH; b binding of NCK1-AS1 and miR-138-2-3p identified using RNA pull-down assay, * compared to the Bio-NC group, p < 0.05; c reaction between NCK1-AS1 and AGO2 identified by RIP assay, * compared to the IgG group, p < 0.05; d-e, mRNA (d) and protein (e) expression of NCK1-AS1, miR-138-2-3p and TRIM24 in cells following NCK1-AS1 and miR-138-2-3p interventions measured using RT-qPCR and western blot analysis, respectively, * compared to the oe-NC group, p < 0.05; ^# compared to the sh-NC group, p < 0.05; ^& compared to the mimic NC group, p < 0.05; ^@ compared to the inhibitor NC group, p < 0.05. Measurement data were measured using mean ± SD; differences among multiple groups were analyzed using one-way ANOVA; Repetition = 3