Fig. 5
Up-regulation of miR-138-2-3p inhibits invasion and migration but promotes apoptosis of U251 cells via down-regulating TRIM24. a proliferation of U251 cells detected using EdU assay; b migration of U251 cells detected using the scratch test and observed under the microscope (× 100); c number of invaded cells in the Transwell assay observed under the microscope (× 100); d-e cell cycle (d) and the apoptosis rate (e) of U251 cells measured using flow cytometry; f, protein levels of Wnt/β-catenin pathway related factor (Wnt1, Wnt3a and β-catenin), proliferation-related factor (PCDA), invasion and migration related factors (N-cadherin and MMP), and apoptosis-related factors (Bcl-2 and Bax) in U251 cells were determined using western blot analysis. * compared to the mimic-NC group, p < 0.05; # compared to the inhibitor-NC group, p < 0.05; & compared to the miR-138-2-3p mimic + oe-NC group, p < 0.05. Measurement data were measured using mean ± SD; differences among multiple groups were analyzed using one-way ANOVA; Repetition = 3