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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Cross-sectional analysis of circulating tumor DNA in primary colorectal cancer at surgery and during post-surgery follow-up by liquid biopsy

Fig. 1

Study flowchart. Tissue and blood samples from CRC patients bearing primary tumors were collected on the day of surgery, and processed to obtain tDNAs and ctDNA, respectively. Lab staff was double-blinded, and all tDNAs were analyzed by two different NGS platforms, e.g. the ThermoFisher Ion Torrent S5 with the ACS targeted NGS panel (a), and the Illumina MySeq with the TST targeted NGS panel (b). Gene panels and their overlap are shown (right). Selected mutations were then validated by the QuantStudio 3D dPCR system (c). Sequencing was by NextSeq also in the case of ctDNAs (d); validation of selected mutations was by dPCR (e)

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