Fig. 4

PICK1 is a direct target of miR-615-3p. a The real-time PCR analysis of PICK1 mRNA level in MCF-7 and T47D cells infected with miR-615-3p-expressing or control vector. b Western blot analysis of PICK1 protein levels in MCF-7 and T47D cells infected with miR-615-3p-expressing or control vector (upper panel). And Western blot analysis of PICK1 protein level in MDA-MB-468 and MDA-MB-231 cells transfected with anti-miR-615-3p or control oligo (lower panel). c Gene structure of PICK1 showing the predicted target site of miR-615-3p in its 3′-UTR. d MCF-7 cells were transfected with a reporter gene containing wild-type (3′UTR-WT) or mutant (3′UTR-Mu) PICK1 3′-UTR along with miR-615-3p or control vector, anti-miR-615-3p or control oligo as indicated in MDA-MB-231 cells