Fig. 5

miR-615-3p overexpression and PICK1 inhibition produce similar changes, which are restored by PICK1 ectopic expression in vitro. (a) Transwell invasion and migration assays of MCF-7 cells were performed after transfection with negative control (NC), siRNA against PICK1, and/or miR-615-3p, PICK1(lacks the 3′UTR) as indicated. (b) Phase contrast microscopy of MCF-7 cells transfected as indicated (scale bars, 50 μm). (c) MCF-7 cells were transfected with PICK1 siRNAs, then PICK1, E-cadherin, Vimentin, TβRI and TβRII protein levels were detected by Western blot analysis (left panel). Western blot analysis was used to determine the effect of PICK1 knockdown on TβRI and TβRII expressions in MCF-7 and T47D cells (right two panels). β-actin served as an internal control