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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Extended half-life target module for sustainable UniCAR T-cell treatment of STn-expressing cancers

Fig. 1

Illustrative representation of the UniCAR system and the αSTn-IgG4 TM construct. a The UniCAR system can be combined with different TM formats, namely scFv- and IgG4-based TMs, to specifically redirect UniCAR T-cells to tumor cells via the E5B9 epitope. UniCAR T-cells are genetically engineered to express the humanized scFv derived from the anti-La mAb 5B9 fused to the transmembrane domain of human CD28 and intracellular signaling domains of CD28 and CD3ζ. b Detailed representation of the αSTn-IgG4 TM construct, comprising the anti-STn scFv (L2A5) arranged in VH - VL orientation linked by a (G4S)3 peptide, the hinge and Fc region of the human IgG4 molecule, and the E5B9 epitope. After expression the αSTn-IgG4 TM will form homodimers due to formation of disulfide bridges between the cysteine residues present in the IgG4 hinge region. c In order to confirm UniCAR expression, genetically modified T-cells were stained with isotype control (upper panel) or anti-La mAb 7B6 (lower panel). Specific binding was detected using a PE-labeled anti-mouse-IgG mAb. Density plots show percentage of CAR surface expression (E7B6) versus expression of co-translated EGFP protein marker for one representative donor. G, glycine; S, serine; A, alanine; P, proline; C, cysteine

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