Fig. 4From: Extended half-life target module for sustainable UniCAR T-cell treatment of STn-expressing cancersCytotoxic potential of retargeted UniCAR T-cells via αSTn-IgG4 TM. Killing of MDA-MB-231 STn+(b) and MCR STn+(a), was determined using standard chromium release assay. Chromium-labeled STn-expressing cell lines were incubated with control T-cells (vector control and UniCAR Stop) or UniCAR 28/ζ T-cells for 24 h at an E:T ratio of 5:1, in the presence or absence of 10 pmol αSTn TM and αSTn-IgG4 TM. c and d Following a similar experimental setup using increasing TM concentrations, half maximal effective concentration (EC50) was determined from the resulting dose-response curves using MDA-MB-231 STn+(c) and MCR STn+(d) cells. Data for three individual donors were summarized as mean specific lysis ± SD. Statistical significance was obtained using 2-way ANOVA with Bonferroni multiple-comparison test (****p < 0.0001, with respect to w/o TM experimental setting)Back to article page