Fig. 5
From: Extended half-life target module for sustainable UniCAR T-cell treatment of STn-expressing cancers
Proinflammatory cytokine secretion profile of UniCAR T-cells redirected via αSTn and αSTn-IgG4 TMs. T-cells transduced with UniCAR 28/ζ constructs were co-cultured with MDA-MB-231 STn+(a) or MCR STn+(b) in the presence or absence of αSTn or αSTn-IgG4 TMs at an E:T ratio of 5:1. After incubation for 24 h, cell culture supernatants were harvested and analyzed using the MACSPlex Cytokine 12 kit. Average cytokine concentrations and SD for three individual donors are shown. Statistical significance was determined using 1-way ANOVA with Bonferroni multiple-comparison test (*p < 0.05; **p < 0.01)