Skip to main content
Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Hsa_circ_0003998 promotes epithelial to mesenchymal transition of hepatocellular carcinoma by sponging miR-143-3p and PCBP1

Fig. 1

The characteristics and expression of the circ0003998. a Cluster heat maps displayed the increase and decrease of circRNAs in 3 HCC patients with PVTT metastasis and 3 HCC patients without PVTT metastasis. Rows indicated circRNAs while columns indicated samples. b The scheme illustrated the production of circ0003998 as well as the results of Sanger sequencing. c The existence of cric0003998 was validated in HCC cells by agarose gel electrophoresis. GAPDH was used as a negative control. d QRT-PCR detected the abundance of circ0003998 and mRNA of ARFGEF2 in HCC cells treated with RNase R, respectively. e QRT-PCR showed the relative expression of circ0003998 in HCC, PVTT, and ANL tissues from cohort 1. f ISH assay detected the expression of circ0003998 in HCC, PVTT, and ANL tissues from cohort 1; and there were 6 missing value. g Semi-quantitative Histochemistry score (H-SCORE) was used to calculate the relative expression of circ0003998 in HCC, PVTT and ANL tissues of TMA-ISH assay. H- SCORE = ∑(percentage of cells of weak intensity× 1) + (percentage of cells of moderate intensity × 2) + (percentage of cells of strong intensity× 3); Relative H-SCORE in PVTT tissues or HCC tissues = (H-SCORE in PVTT tissues or HCC tissues)/(H-SCORE in ANL tissues). h QRT-PCR showed the relative expression of circ0003998 in HCC cell lines. i The volcano plot showed the expression profile of mRNAs between HCC-OE cells and HCC-NC cells. j The Gene Ontology (GO) functional pathways that mRNAs enriched on were showed by Chord Layout. k Western blot showed the protein level of EMT markers after over expression or silencing of circ0003998. Data were presented as means ± SD. Student’s t-test was used. *p-value< 0.05, **p-value< 0.01, ***p-value< 0.001. convergent primer; divergent primer

Back to article page