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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Hsa_circ_0003998 promotes epithelial to mesenchymal transition of hepatocellular carcinoma by sponging miR-143-3p and PCBP1

Fig. 5

Circ0003998 promoted the EMT in HCC cells through PCBP1/CD44v6 pathway. a Protein pull-down experiment was performed using the circ0003998-specific probe and control probe; and the enrichment of protein was detected by the protein spectrum. b RIP experiment was performed with anti-PCBP1 antibody and negative control IgG, followed by qRT-PCR to detect the expression of circ0003998. c FISH was performed to observe the cellular location of circ0003998 (red) and PCBP1 (green) in HepG2 cells (magnification, × 400, scale bar, 25 μm). d-e The binding sites between circ0003998 and PCBP1 were predicted online. f The structure of the KH1 of PCBP1 was modeled. g-h Rescue transwell assay was performed after transfection with indicated vectors, OE-PCBP1 or si-PCBP1 (magnification, × 100, scale bar, 100 μm). i GO analysis showed the enriched pathway of PCBP1 target genes. j-k QRT-PCR showed the mRNA level of the targets of PCBP1 after over expression or silencing of circ0003998. l Western blot showed the protein level of the CD44v6 after over expression or silencing of circ0003998. m-n Relative expression of CD44v6 was detected in circ0003998-OE HepG2 cells transfected with OE-PCBP1 and circ0003998-silencing HepG2 cells transfected with si-PCBP1. o QRT-PCR detected the relative mRNA levels of CD44v6 in HCC, PVTT, and ANL tissues in cohort 1. p The correlation between the expression of circ0003998 and CD44v6 was analyzed in HCC tissues and PVTT tissues in cohort 1. The correlation was measured by Pearson correlation analysis. Data are presented as means ± SD; n = 3. Student’s t-test was used. *p < 0.05, **p < 0.01, ***p < 0.001

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