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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: SH3BGRL confers innate drug resistance in breast cancer by stabilizing HER2 activation on cell membrane

Fig. 2

SH3BGRL prolongs the duration of HER2 on cell membrane. a Effects of EGF stimulation on intracellular distribution of HER2. Under EGF stimulation (20 ng/mL), HER2 (red) was detected in MDA-MB-453 cells by immunofluorescence assay. Left: EGF stimulated 5 min, Right: EGF stimulated 30 min. Arrows point the HER2 location. Doted boxes show the overall HER2 location states after EGF treatment. b Immunoblots of the subcellular fractioned HER2. With EGF stimulation (20 ng/mL), cells were harvested, SH3BGRL and HER2 in cell membrane (M) and cytoplasm (C) fractions were detected. c Co-localization of HER2 with the endocytosis-related protein Caveolin-1 in MDA-MB-453 cells by confocal analysis. Cells were stained with Caveolin-1 (orange), HER2 antibody (red), and the merged images are shown on the right. Arrows point the HER2 and Caveolin-1 locations

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