Fig. 6

The p38MAPK and PI3K-AKT signaling pathways are involved in the regulation of p21 by GLYR1. a Co-immunoprecipitation assays to validate the interaction between GLYR1 and p38 in SW480 and SW620 cells. b Western blot analysis of PI3K, p-PI3K, AKT, p-AKT, P38, p-p38, and p21 in SW480-siGLYR1 and SW620-siGLYR1 cells. The expression of p-PI3K and p-AKT was upregulated in the siGLYR1 groups compared with the siNC cells; total protein was used as an internal control. Error bars represent the mean ± SD (n = 3), *P < 0.05, **P < 0.01, ***P < 0.001. c Molecular mechanism of the function of GLYR1 downregulation in MSI CRC