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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: CPI-613 rewires lipid metabolism to enhance pancreatic cancer apoptosis via the AMPK-ACC signaling

Fig. 5

Blockade or enhancement of autophagy has a marginal effect on CPI-613-induced apoptosis in pancreatic cancer cells. a-d The effects of the autophagy inhibitor CQ on CPI-613-induced apoptosis. AsPC-1 and PANC-1 cells were treated with or without 200 μM CPI-613 for 48 h in the presence or absence of 20 μM CQ, and apoptosis was determined by flow cytometry with Annexin V-FITC/PI staining (a, b) and Western blot with antibody against PARP (c). d The effects of CQ on CPI-613-induced cell viability determined by MTT assays. e-h The effects of the autophagy inducer simvastatin on CPI-613-induced apoptosis. AsPC-1 and PANC-1 cells were treated with or without 200 μM CPI-613 for 48 h in the presence or absence of 10 μM simvastatin, and apoptosis was determined by flow cytometry with Annexin V-FITC/PI staining (e, f) and Western blot with antibody against PARP (g). h The effects of simvastatin on CPI-613-induced cell viability determined by MTT assays. The representative results of flow cytometry and quantitative data from three independent experiments were shown in (a, e) and (b, f), respectively. n.s.: not significant

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