Fig. 7

The AMPK-ACC signaling contributes to CPI-613-induced apoptosis in pancreatic cancer cells. a, b, e The effects of AMPK inhibitor Compound C on CPI-613-induced apoptosis. AsPC-1 and PANC-1 cells were treated 10 μM Compound C for 1 h, followed by treated with 200 μM CPI-613 for 48 h. Apoptosis was determined by flow cytometry with Annexin V-FITC/PI staining (a, b) and western blot with antibodies against PARP and p-ACC (e). c, d, f The effects of ACC inhibitor TOFA on CPI-613-induced apoptosis. AsPC-1 and PANC-1 cells were treated with 10 μM TOFA for 1 h, followed by treated with 200 μM CPI-613 for 48 h. Apoptosis was determined by flow cytometry with Annexin V-FITC/PI staining (c, d) and Western blot with antibodies against PARP and p-ACC (f). The representative results of flow cytometry and quantitative data from three independent experiments were shown in (a, c) and (b, d), respectively. (g) Schematic representation of the mechanism of CPI-613 action in pancreatic cancer cells. *p < 0.05