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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Sur-X, a novel peptide, kills colorectal cancer cells by targeting survivin-XIAP complex

Fig. 1

Peptide Sur-X targeted the survivin-XIAP complex in colorectal cancer cells. a The expression of IAPs in all cancer types available in Oncomine. b Analysis of survivin and XIAP expression in colorectal cancer by GEPIA (T = 275, N = 349). N, normal; T, tumor; *, p < 0.05. c The survivin-XIAP interaction in human colorectal cancer cells HCT116 and RKO, as determined by co-immunoprecipitation. Anti-Sur, anti-survivin antibody; anti-XIAP, anti-XIAP antibody. Three independent experiments were performed. d The amino acid sequence of Sur-X. Residues from TAT were labeled with green, those from survivin were labeled with orange. e Intracellular localization of FITC-Sur-X and FITC-Con. Scale bar, 20 μm. Three independent experiments were performed. f Effect of Sur-X on survivin-XIAP interaction as determined by co-immunoprecipitation. HCT116 cells were treated by 10 μM Sur-X for 1 h or not. Co-immunoprecipitation was performed by anti-XIAP antibody (left pannel) and anti-survivin antibody (right pannel), respectively. Survivin and XIAP were detected by Western blot analysis. GAPDH was used as a loading control. Anti-Sur, anti-survivin antibody; anti-XIAP, anti-XIAP antibody. Three independent experiments were performed

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