Fig. 2
From: EVI5 is an oncogene that regulates the proliferation and metastasis of NSCLC cells
Knockdown of EVI5 inhibits cell proliferation, migration and invasion in vitro. a EVI5 mRNA and protein levels in EVI5-knockdown NSCLC cells. b CCK-8 assay of cell viability in A549 and H226 cells (si-EVI5 compared with si-NC). c Flow cytometry assay of A549 and H226 cells (si-EVI5 compared with si-NC). Cells were harvested 72 h after transfection and stained with PI. The percentage of cells in each cell cycle phase is shown in the inset of each panel. d Wound healing assay was performed to evaluate cell migration in A549 and H226 cells (si-EVI5 compared with si-NC). e Representative images of the transwell assay results for cell migration and invasion in A549 and H226 cells (si-EVI5 compared with si-NC). β-actin was used as the internal control. Bars represent mean ± SD from three independent experiments. Significant differences compared with the control: * P < 0.05; **P < 0.01; ***P < 0.001