Fig. 5

EVI5 mediates the migration and invasion of NSCLC cells through the TGF-β/Smad signaling pathway. a Data obtained from several study groups deposited in the GEPIA2 database were analysed to explore the correlation between EVI5 and TGF-β receptors mRNA levels. b The levels of TGF-β receptor II, TGF-β receptor I, p-Smad3, Snail, N-cadherin, and MMP2 were significantly decreased and E-Cadherin was significantly increased in EVI5-knockdown cells compared with control cells. c Co-immunoprecipitation of EVI5 and TGF-β receptor II were shown, protein were immunoprecipitated from lysates of control and EVI5-overexpressing or EVI5-KO A549 cells using a specific monoclonal antibody. d EVI5-KO A549 and H226 cells treated or untreated with TGF-β1 (5 ng/ml) were allowed to migrate through 8-mm pore size transwell inserts. One day later, migratory and invasive cells were stained and counted in at least three light microscopic field, One representative image is shown (Cas-9 group compared with EVI5-KO group, Cas-9 + TGF-β1 group compared with EVI5-KO + TGF-β1 group). e EVI5-KO A549 cells were treated with TGF-β1 (5 ng/ml) for 24 h, and the expression levels of various proteins were then measured by western blot analysis. β-actin was used as the internal control. Bars represent mean ± SD from three independent experiments. Significant differences compared with the control:***P < 0.001