Fig. 4
MSI2a negatively regulates TNBC cell growth and migration in vivo. a Western blot assays of SLUG, ZO-1, vimentin, E-cadherin, N-cadherin, β-catenin, p-ERK1/2, and ERK1/2 expression in MDA-MB-231 and Hs-578T cells. b Tumorigenicity assay of MSI2a-overexpressing and -b-overexpressing MDA-MB-231 cells (2 × 106) after subcutaneous injection in the flanks of nude mice (n = 4). The mice and tumors are shown. The quantification of tumor volume is shown on the right side. Scale bar, 1 cm. c Representative images of nude mouse lungs detecting MDA-MB-231 cells at 50 days after tumor cells were injected into the tail vein (scale bar, 1 cm) and H&E staining of the lungs (right; scale bar, 100 mm); arrows, metastatic foci. d Statistical analysis of the numbers of lung surface metastases using Student’s t test. n = 5. e H&E and MSI2 and Ki67 staining of xenograft tissues of mice injected with MSI2a-overexpressing MDA-MB-231 cells. Scale bar, 100 μm. *p < 0.05, **p < 0.01, ***p < 0.001