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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Different p53 genotypes regulating different phosphorylation sites and subcellular location of CDC25C associated with the formation of polyploid giant cancer cells

Fig. 2

CDC25C, cyclin B1, and CDK1 expression in HEY, SKOv3, BT-549 and MDA-MB-231 cells before and after CoCl2 treatment. a Western blot showed the total protein expression of CDC25C, cyclin B1, and CDK1 in HEY, SKOv3, BT-549 and MDA-MB-231 control and PGCCs with daughter cells. b Western blot results show CDC25C, cyclinB1, and CDK1 cytoplasmic and nuclear expression in HEY and BT-549 control and PGCCs with daughter cells. c Total protein expression of CDC25C, cyclin B1, and CDK1 in HEY control and PGCCs with daughter cells with siRNA CDC25C-1103, 1343, 1531, siRNA control, and negative control transfection. d CDC25C, cyclin B1 and CDK1 cytoplasmic and nuclear expression in HEY and BT-549 control and PGCCs with daughter cells with siRNA CDC25C-1531, siRNA control, and negative control transfection. e Histogram was used to quantify wound-healing index in HEY and BT-549 cells by measuring no less than three different healing areas. (a) HEY control cells treated with CDC25C-siRNA and siRNA control. (b) HEY PGCCs and daughter cells treated with CDC25C-siRNA and siRNA control. (c) BT-549 control cells treated with CDC25C-siRNA and siRNA control. (d) BT-549 PGCCs and daughter cells treated with CDC25C-siRNA and siRNA control. f Column diagram of the average cell number for migration and invasion assay in HEY and BT-549 control, PGCCs, and daughter cells after the CDC25C-siRNA and siRNA control transfection. Average cell number for migration among (a) HEY and (b) BT-549 cells after the CDC25C-siRNA and siRNA control transfection. Average cell number for invasion among (c) HEY and (d) BT-549 cells after the CDC25C-siRNA and siRNA control transfection. g Column diagram shows the colony formation efficiency in HEY and BT-549 control and PGCCs with daughter cells after CDC25C-siRNA and siRNA control transfection. Colony formation efficiency of (a) HEY and (b) BT-549 with different treatment. Treatment: Cells treated with CoCl2. CDC25i: CDC25C-1531 siRNA

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