Fig. 6

MiR-374b-5p directly targets ABCA8 and is correlated with poor prognosis. a Binding site of miR-374b-5p in wild-type (WT) 3′-UTR of ABCA8 and corresponding mutant type were constructed as shown on the left. The Luciferase reporter assay showed luciferase activity of HEK293T transfected with WT 3′-UTR was inhibited by miR-374b-5p overexpression. b Human HCC tissues displayed significantly higher miR-374b-5p levels than adjacent non-tumor tissues 105 patients. c Kaplan-Meier analysis indicated that the combination of low ABCA8 and high miR-374-5p predicts a poorer overall survival rate than high ABCA8 and low miR-374b-5p. d MiR-374-5p silencing increased ABCA8 protein levels and miR-374-5p overexpression decreased ABCA8 in indicated cells. Data are means ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001