Fig. 1

Identification of circ_0008532 in bladder cancer. a-b The Venn and schematic diagrams show that five exons derived from MTGR1 constitute circ_0008532. The existence of circ_0008532 was demonstrated by PCR and its back-splicing junction was verified by Sanger sequencing. c The expression level of Circ_0008532 in 10 pairs of bladder cancer and adjacent non-tumor tissue. d The expression level of Circ_0008532 in normal bladder urothelial cells (NBUCs), human uroepithelial cells (SV-HUC-1) and bladder cancer cell lines (EJ, T24T, UM-UC-3, TCCSUP, T24, 5637, SCABER, SW780 and J82). e PCR assay with divergent or convergent primers indicating circ_0008532 is present in the EJ and T24 cell lines. GAPDH was used as a negative control. f Real-time qPCR analysis of the expression of circ_0008532 after RNase R treatment in EJ or T24 cells. g RNA-FISH indicates the location of circ_0008532. Nuclei were stained blue with DAPI. Circ_0008532 is stained red with cy3. Bar graphs show the statistical analysis of three independent experiments (* P < 0.05)