Fig. 2

Circ_0008532 promotes BC cell migration, invasion, angiogenesis, and tumor metastasis. a Schematic representation of the sites of the siRNA specific to the back-splice junction of circ_0008532. b The level of circ_0008532 in EJ and T24 cells with stable expression or silencing of circ_0008532. c Representative pictures (left panel) and quantification (right panel) of invaded cells analyzed using a Transwell Matrigel assay. d Representative pictures (left panel) and percentage of the original wound area (right panel) of EJ cells analyzed using a wound-healing assay. e Representative images (left panel) and quantification (right panel) of HUVECs cultured on Matrigel-coated plates with conditioned medium from EJ cells. f Representative image of metastatic lung colonization in nude mice . g Kaplan–Meier curves for nude mice. Bar graphs show the statistical analysis of three independent experiments (* P < 0.05)