Fig. 2

Xanthohumol (XN) suppresses OSCC cells. a Xanthohumol is a candidate natural product which inhibits OSCC cell viability significantly. A total of 70 compounds (1 μM) of interest were treated with CAL27 cells for 48 h. Cell viability was examined by the MTS assay. b The chemical structure of xanthohumol. c MTS assay analysis of the effect of xanthohumol on immortalized HaCat cells. d The effect of xanthohumol on cell viability of OSCC cells. SCC25 (left), SCC9 (middle), and CAL27 (right) cells were treated with xanthohumol or DMSO control. Cell viability was examined by the MTS assay. e The effect of xanthohumol on colony formation of OSCC cells. SCC25 (top), SCC9 (middle), and CAL27 (bottom) cells were treated with xanthohumol or DMSO control, the colony formation efficacy of OSCC cells was determined by soft agar assay. XN, xanthohumol. *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar, 200 μm