Fig. 1

Selection of circ-001971 (a) Pathological characteristics of the tumor and non-cancerous tissues revealed by H&E staining. b The expression of the top 11 upregulated circRNAs reported by the previous study was verified in 13 paired tumor and noncancerous tissues using real-time PCR. The data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01. c The expression of circ-001971 was examined in 70 paired tumor and noncancerous tissues using real-time PCR. d Circ-001971 expression was analyzed according to the TNM stage. e ROC curve of circ-001971 to distinguish colorectal cancer (CRC) from controls. f Colorectal cancer (CRC) cases were divided into two groups according to circ-001971 expression. Overall survival was analyzed by Kaplan-Meier overall survival analysis using the log-rank test. g ROC curve of circ-001971 to assess the diagnostic sensitivity and specificity of circ-001971 for CRC