Fig. 3

Effects of circ-001971 on CRC growth in vivo (a) Lentivirus containing shRNA targeting circ-001971 was constructed and the knockdown efficiency was verified by real-time PCR. b Six animals were randomly assigned into two groups (n = 3). SW620 cells infected with Lv-sh-NC or Lv-sh-circ-001971 were hypodermically injected into the left axillaries of mice in different groups. Twenty-five days after the injection, when the diameters of the resulting tumors measured approximately 5 mm, the rats were sacrificed under anesthesia. c The length (L) and width (W) of the tumors were measured with calipers every 5 days for 25 days and the tumor volumes were calculated. d At the end of the animal experiments, all the animals were sacrificed under anesthesia, and the tumor weight was determined. **P < 0.01, compared to Lv-sh-NC group