Fig. 6

Synergistic effect of DCZ3301 and BTZ against BTZ-resistant MM in vivo. (a) Gross appearance of tumors on day 20. (b) Tumor growth curves of 20 days treatment. (c) Growth curve of mouse weight (n = 3 for each group). (d) and (e) Serum levels of ALT, AST, Cr and BUN (n = 6 for each group). *p < 0.05, #p > 0.05 Data were represented as mean ± SD. (f) H&E staining of tumor sections for tumor histology after treatment. TUNEL, Ki-67, γ-H2A.X, cleaved caspase-3, phospho-ATM and phospho-CHK1 were stained immunohistochemically in tumor sections. (g) The percentage of cell shrinkage and TUNEL-positive cells in tumor sections. (h) The relative protein expressions of Ki-67, γ-H2A.X, cleaved caspase-3, phospho-ATM and phospho-CHK1 quantified by Image Pro-plus in tumor sections