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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Transcription activation of circ-STAT3 induced by Gli2 promotes the progression of hepatoblastoma via acting as a sponge for miR-29a/b/c-3p to upregulate STAT3/Gli2

Fig. 4

STAT3 partially rescued the effects of circ-STAT3 on cell biological functions. a Overexpression efficiency of STAT3 was assessed via qRT-PCR. Student’s t-test. b-c EdU (scale bar: 100 μm) and colony formation assay detected influence of STAT3 overexpression on circ-STAT3 depletion-mediated proliferation ability of HB cells. One-way ANOVA. d-e Transwell (scale bar: 200 μm) and wound healing assay (scale bar: 200 μm) examined cell invasion and migration ability in sh-NC, sh-circ-STAT3#2 and sh-circ-STAT3#2+ pcDNA3.1/STAT3 group. One-way ANOVA. f-h Sphere formation assay (scale bar: 200 μm), qRT-PCR and western blot analysis revealed cell stemness in sh-NC, sh-circ-STAT3#2 and sh-circ-STAT3#2+ pcDNA3.1/STAT3 group. One-way ANOVA. *P < 0.05, **P < 0.01

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