Fig. 3

LINC00520 promotes the proliferation, invasion and migration of melanoma cell. a Transfection efficiency of LINC00520 siRNA was determined by PCR. b The proliferative ability of melanoma cells was determined by CCK8 assay in different groups. c The DNA synthesis of melanoma cells grown was detected by EdU assay after transfection with NC or LINC00520 siRNA. Scale bar, 100 μm. d Western blots identified N-cadherin, E-cadherin and Vimentin protein expression changes in NC or LINC00520 siRNA transfected melanoma cells, GAPDH was used as a control. e The invasive capacity of NC or LINC00520 siRNA transfected melanoma cells was assessed by transwell assay. Scale bar, 50 μm. f Migration of melanoma cells in different transfection groups was detected by scratch wound assay. Scale bar, 100 μm. Data were expressed as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001