Fig. 1

Relative expression of miR-550a-3-5p in HPV-positive OSCC tissue samples. a Result of an Agilent human miRNA microarray showed differentially-expressed miRNAs between HPV-positive (n = 3) and HPV-negative (n = 3) OSCC tissue samples (Fold change ≥2, P<0.05). b Relative expressions of miR-550a-3-5p, miR-451a, miR-210-3p in HPV-positive (n = 25), HPV-negative (n = 45) OSCC tissue samples and normal oral mucosa (n = 20) were evaluated by qRT-PCR. c Representative FISH images of miR-550a-3-5p in HPV-positive, HPV-negative OSCC tissue samples and normal oral mucosa using formalin-fixed paraffin-embedded sections. H&E staining and IHC staining of p16 were used to confirm tissue types and HPV infection status (magnification 200X and 400X). d Kaplan-Meier survival analysis of patients with (1) HPV-positive and HPV-negative OSCC, (2) higher and lower miR-550a-3-5p expression in HPV-negative OSCC, and (3) higher and lower miR-550a-3-5p expression in HPV-positive OSCC. Quantitative analysis based on triplicate experiments. Results were presented as means ± SD. *P<0.05, **P<0.01, ***P<0.001