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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: RETRACTED ARTICLE: Interplay between cancer cells and M2 macrophages is necessary for miR-550a-3-5p down-regulation-mediated HPV-positive OSCC progression

Fig. 4

miR-550a-3-5p inhibited M2 macrophages polarization to suppress migration, invasion, and EMT of HPV-positive OSCC cells. a Schema for representing the experiment procedures. b PMA-induced THP-1 cells were cultured with HPV-positive OSCC cells (UPCI:SCC090 and UM-SCC-47)-conditioned media for 24 h, and morphology was observed using FITC-phalloidin staining (green). Nuclei were stained using DAPI (blue) and PMA-THP-1 cells were used as controls (magnification 200X). c qRT-PCR analyzed the expression of the markers of pan-macrophage (CD68), M1 (CD80, IL-12p40, TNF-α) and M2 (CD163, IL-10, Arginase-1) macrophages in PMA-THP-1 cells incubated with CM from miR-550a-3-5p-overexpressed HPV-positive OSCC cells for 24 h. d Migration and invasion capacity of miR-550a-3-5p-overexpressed HPV-positive OSCC cells co-cultured with their corresponding TAMs for 48 h were determined by Wound healing and Transwell invasion assay, respectively. Shown were representative images on the upper side with quantitative analysis below (magnification 200X). e qRT-PCR for analyzing mRNA levels of E-cadherin, N-cadherin, β-catenin and Vimentin, Western blot for analyzing protein levels of E-cadherin and Vimentin in miR-550a-3-5p-overexpressed HPV-positive OSCC cells co-cultured with their corresponding TAMs for 48 h. All assays were carried out in triplicate. Results were presented as means ± SD. *P<0.05, **P<0.01, ***P<0.001

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